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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Parachlamydia acanthamoebae ： ウィキペディア英語版 Parachlamydia acanthamoebae ''Parachlamydia acanthamoebae'' are bacterium that fall into the category of host-associated microorganisms.〔 This bacterium lives within free-living amoebae that are an intricate part of their reproduction. Originally named ''Candidatus Parachlamydia acanthamoebae'', its current scientific name was introduced shortly after.〔 This species has shown to have over eighty percent 16S rRNA gene sequencing identity with the class Chlamydiae. ''Parachlamydia acanthamoebae'' has the same family as the genus ''Neochlamydia'' with which it shares many similarities.〔〔 ==Discovery== The isolation of ''Parachlamydia acanthamoebae'' is credited to Rolf Michel and Barbel Hauroder-Philippczyk in Berlin in 1994. Using a nasal swab from volunteers, they were able to isolate coccoid-shaped bacteria that were present among other naturally-produced organisms. Although at least ten subsequent attempts at reisolation were tried, ''P. acanthamoebae'' was not isolated again until 1997 when the researchers Rudolf Amann, Nina Springer, and Wolfgang Ludwig isolated it with a strain of ''Acanthamoeba'' species. 〔 This sample was transferred to a non-nutrient agar plate and the parasitized trophozoite of the ''Acanthamoeba'' species multiplied.〔 A trophozoite is an active stage in the ''Acanthamoeba'' species life cycle in which the protozoan grows and feeds.〔 The researchers were able to isolate the infected trophozoites by this method only once, as subsequent tries were unsuccessful.〔 The researchers then took small aliquots of the organism and filtered it to ensure the sample was pure.〔 After running these samples through a centrifuge, a machine with a rapid rotating inner container, the samples were used to directly amplify the 16S rRNA gene to form a nearly full-length rRNA sequence by Polymerase Chain Reaction.〔 The ribosomal DNA was sequenced using the T7 sequencing kit of Pharmacia.〔 After analyzing the genome and confirming 86% 16S rRNA gene sequence identity to members of the genus ''Chlamydia'', they proposed ''Parachlamydia acanthamoebae'' be classified under the order of Chlamydiales.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Parachlamydia acanthamoebae」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.